Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10975 | 3' | -58.6 | NC_002794.1 | + | 72831 | 0.69 | 0.697553 |
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Target: 5'- gACGgacGGCAGAcCaCCCGAGAUCCgCGGcUCu -3' miRNA: 3'- -UGC---CCGUCUaG-GGGCUCUAGG-GCU-AG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 145327 | 0.69 | 0.687822 |
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Target: 5'- gGCGGGCGGcgCucgggCCCGAGcgggCCCGAg- -3' miRNA: 3'- -UGCCCGUCuaG-----GGGCUCua--GGGCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 40651 | 0.7 | 0.648546 |
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Target: 5'- aGCGGGCAGcgacguaCUCG-GAUUCCGGUCg -3' miRNA: 3'- -UGCCCGUCuag----GGGCuCUAGGGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 82191 | 0.7 | 0.638677 |
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Target: 5'- cCGGGCg---CCCCGAGcgCCCGGc- -3' miRNA: 3'- uGCCCGucuaGGGGCUCuaGGGCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 49945 | 0.7 | 0.628804 |
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Target: 5'- gACGGGCAGGcgCUCCc-GGUCgCCGGUCg -3' miRNA: 3'- -UGCCCGUCUa-GGGGcuCUAG-GGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 153054 | 0.73 | 0.484488 |
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Target: 5'- uCGGGCGGAcggUCCCCGAGcga-CGGUCg -3' miRNA: 3'- uGCCCGUCU---AGGGGCUCuaggGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 150450 | 0.74 | 0.448519 |
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Target: 5'- gGCGGGUgcGGAUCCCgGAaGGcUCCCGGUUc -3' miRNA: 3'- -UGCCCG--UCUAGGGgCU-CU-AGGGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 48487 | 0.74 | 0.439758 |
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Target: 5'- cCGGGCAGcgCCCaCGAcacguucaGcgCCCGAUCg -3' miRNA: 3'- uGCCCGUCuaGGG-GCU--------CuaGGGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 41303 | 0.78 | 0.256355 |
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Target: 5'- cGCGGGCcgcgugGGGUCUCCGAGGUCuCCGAg- -3' miRNA: 3'- -UGCCCG------UCUAGGGGCUCUAG-GGCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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