Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10975 | 3' | -58.6 | NC_002794.1 | + | 4245 | 0.66 | 0.85609 |
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Target: 5'- uCGGuGCGGcUCUCgGuGGUCUCGAUCg -3' miRNA: 3'- uGCC-CGUCuAGGGgCuCUAGGGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 176958 | 0.66 | 0.85609 |
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Target: 5'- -gGGGCAGcgggacCUCCGGGuAUCCCGGg- -3' miRNA: 3'- ugCCCGUCua----GGGGCUC-UAGGGCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 112294 | 0.67 | 0.824696 |
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Target: 5'- uCGGGCGGccCCCCGGG--UCCGAcuUCg -3' miRNA: 3'- uGCCCGUCuaGGGGCUCuaGGGCU--AG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 66391 | 0.67 | 0.824696 |
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Target: 5'- cGCGGGC---UCCCCGucGUCCCGc-- -3' miRNA: 3'- -UGCCCGucuAGGGGCucUAGGGCuag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 8955 | 0.67 | 0.816424 |
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Target: 5'- gGCGGGCAcaaccucuuaccGAUCUaCGAGGUCCCu--- -3' miRNA: 3'- -UGCCCGU------------CUAGGgGCUCUAGGGcuag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 145542 | 0.67 | 0.816424 |
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Target: 5'- uCGGGCg---CCCCGAGcggCCCGAc- -3' miRNA: 3'- uGCCCGucuaGGGGCUCua-GGGCUag -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 137904 | 0.67 | 0.807995 |
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Target: 5'- gACGGuacaCAGGUCCCCGuGGcguUCgCGGUCg -3' miRNA: 3'- -UGCCc---GUCUAGGGGCuCU---AGgGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 146707 | 0.67 | 0.807995 |
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Target: 5'- uGCGGGagcuGGuggCCCUGcuGGAUCUCGAUCu -3' miRNA: 3'- -UGCCCg---UCua-GGGGC--UCUAGGGCUAG- -5' |
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| 10975 | 3' | -58.6 | NC_002794.1 | + | 41303 | 0.78 | 0.256355 |
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Target: 5'- cGCGGGCcgcgugGGGUCUCCGAGGUCuCCGAg- -3' miRNA: 3'- -UGCCCG------UCUAGGGGCUCUAG-GGCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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