Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10979 | 3' | -50.7 | NC_002794.1 | + | 114257 | 0.66 | 0.997352 |
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Target: 5'- -----aAGAGCGUCUCcGGGggcgggucguGCUGCACc -3' miRNA: 3'- caagucUCUCGCAGAG-CCU----------UGAUGUG- -5' |
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| 10979 | 3' | -50.7 | NC_002794.1 | + | 113718 | 0.66 | 0.996872 |
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Target: 5'- -cUCAGGGAcgGCG--UCGGcGACUGCACc -3' miRNA: 3'- caAGUCUCU--CGCagAGCC-UUGAUGUG- -5' |
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| 10979 | 3' | -50.7 | NC_002794.1 | + | 105018 | 0.67 | 0.991115 |
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Target: 5'- ---gAGGGAGCGga--GGAugUGCACg -3' miRNA: 3'- caagUCUCUCGCagagCCUugAUGUG- -5' |
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| 10979 | 3' | -50.7 | NC_002794.1 | + | 94294 | 0.69 | 0.977692 |
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Target: 5'- --gCGGGGGGCGgCUCGGGcgcgccgaugcaaggACUGCAUc -3' miRNA: 3'- caaGUCUCUCGCaGAGCCU---------------UGAUGUG- -5' |
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| 10979 | 3' | -50.7 | NC_002794.1 | + | 19507 | 0.69 | 0.976708 |
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Target: 5'- --gCGGAGGucGCGccgCUCGGAGCgccgGCGCg -3' miRNA: 3'- caaGUCUCU--CGCa--GAGCCUUGa---UGUG- -5' |
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| 10979 | 3' | -50.7 | NC_002794.1 | + | 97195 | 0.69 | 0.971309 |
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Target: 5'- -aUCAGAagGAGCuGUUUuuccaaCGGAGCUACGCu -3' miRNA: 3'- caAGUCU--CUCG-CAGA------GCCUUGAUGUG- -5' |
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| 10979 | 3' | -50.7 | NC_002794.1 | + | 147783 | 0.74 | 0.815763 |
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Target: 5'- --gCGGcgacccuGGGCGUCUCGGAGCUgACGCu -3' miRNA: 3'- caaGUCu------CUCGCAGAGCCUUGA-UGUG- -5' |
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| 10979 | 3' | -50.7 | NC_002794.1 | + | 135390 | 0.88 | 0.210472 |
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Target: 5'- -cUCAGAGAGCGUCUCGGAA--ACGCu -3' miRNA: 3'- caAGUCUCUCGCAGAGCCUUgaUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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