Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10992 | 5' | -50.6 | NC_002794.1 | + | 11961 | 0.71 | 0.957059 |
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Target: 5'- -cUCCgUCUacgCuCGUGGAGAUGGCGUc -3' miRNA: 3'- caAGG-AGAaaaG-GCGCCUCUACUGCG- -5' |
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| 10992 | 5' | -50.6 | NC_002794.1 | + | 139577 | 0.71 | 0.953117 |
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Target: 5'- uGUUUUg--UUUCCgGCGGuGGAUGACGCg -3' miRNA: 3'- -CAAGGagaAAAGG-CGCC-UCUACUGCG- -5' |
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| 10992 | 5' | -50.6 | NC_002794.1 | + | 110051 | 0.71 | 0.948939 |
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Target: 5'- -gUCCUCgcggaUCCGuCGGAGA--GCGCg -3' miRNA: 3'- caAGGAGaaa--AGGC-GCCUCUacUGCG- -5' |
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| 10992 | 5' | -50.6 | NC_002794.1 | + | 129538 | 0.72 | 0.92767 |
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Target: 5'- gGUUCCUCUUUUCCGCGucaucucCGCg -3' miRNA: 3'- -CAAGGAGAAAAGGCGCcucuacuGCG- -5' |
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| 10992 | 5' | -50.6 | NC_002794.1 | + | 69354 | 0.73 | 0.90672 |
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Target: 5'- --aUCUCguuccCCGCGGAGAUcgGGCGCg -3' miRNA: 3'- caaGGAGaaaa-GGCGCCUCUA--CUGCG- -5' |
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| 10992 | 5' | -50.6 | NC_002794.1 | + | 33026 | 0.75 | 0.806424 |
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Target: 5'- --aCCUCgacgcCCGCGGAGccGACGCc -3' miRNA: 3'- caaGGAGaaaa-GGCGCCUCuaCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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