Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10992 | 5' | -50.6 | NC_002794.1 | + | 145596 | 0.66 | 0.997167 |
Target: 5'- --gCCggCUc--CCGCGGAGucgGGCGCg -3' miRNA: 3'- caaGGa-GAaaaGGCGCCUCua-CUGCG- -5' |
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10992 | 5' | -50.6 | NC_002794.1 | + | 12319 | 0.66 | 0.997167 |
Target: 5'- -cUCCUCcgcggUCCGCgugaccguGGAGAcgGACGUc -3' miRNA: 3'- caAGGAGaaa--AGGCG--------CCUCUa-CUGCG- -5' |
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10992 | 5' | -50.6 | NC_002794.1 | + | 185214 | 0.67 | 0.995433 |
Target: 5'- ---gCUCgcg-CCGCGGAGAgucGCGCc -3' miRNA: 3'- caagGAGaaaaGGCGCCUCUac-UGCG- -5' |
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10992 | 5' | -50.6 | NC_002794.1 | + | 179106 | 0.66 | 0.996663 |
Target: 5'- -gUCCggucgUCCGCGGuccgGGCGCg -3' miRNA: 3'- caAGGagaaaAGGCGCCucuaCUGCG- -5' |
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10992 | 5' | -50.6 | NC_002794.1 | + | 41233 | 0.67 | 0.995433 |
Target: 5'- aUUCCUCccacgCCGCGGccGccGACGCc -3' miRNA: 3'- cAAGGAGaaaa-GGCGCCu-CuaCUGCG- -5' |
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10992 | 5' | -50.6 | NC_002794.1 | + | 69354 | 0.73 | 0.90672 |
Target: 5'- --aUCUCguuccCCGCGGAGAUcgGGCGCg -3' miRNA: 3'- caaGGAGaaaa-GGCGCCUCUA--CUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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