Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10993 | 3' | -55 | NC_002794.1 | + | 146936 | 0.66 | 0.948446 |
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Target: 5'- --gAGAGCGCUCGG-UCUG-GCguGGc -3' miRNA: 3'- cgaUCUCGCGAGCCuAGACgUGguCU- -5' |
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| 10993 | 3' | -55 | NC_002794.1 | + | 16700 | 0.66 | 0.952564 |
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Target: 5'- cGCUcgcGAGuCGCUCgccguccgaGGAgUCUGCGCCGGc -3' miRNA: 3'- -CGAu--CUC-GCGAG---------CCU-AGACGUGGUCu -5' |
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| 10993 | 3' | -55 | NC_002794.1 | + | 187461 | 0.66 | 0.952564 |
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Target: 5'- cGCUcGGcGuCGCUCGGcgC-GCGCCGGGc -3' miRNA: 3'- -CGA-UCuC-GCGAGCCuaGaCGUGGUCU- -5' |
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| 10993 | 3' | -55 | NC_002794.1 | + | 114257 | 0.66 | 0.952564 |
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Target: 5'- --aAGAGCGuCUcCGGGggcgggucgugCUGCACCAGc -3' miRNA: 3'- cgaUCUCGC-GA-GCCUa----------GACGUGGUCu -5' |
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| 10993 | 3' | -55 | NC_002794.1 | + | 130151 | 0.66 | 0.956454 |
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Target: 5'- gGCgcGAGCGCg-GGAUCacgcGCGCgAGAg -3' miRNA: 3'- -CGauCUCGCGagCCUAGa---CGUGgUCU- -5' |
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| 10993 | 3' | -55 | NC_002794.1 | + | 41682 | 0.66 | 0.960122 |
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Target: 5'- cGCaGGAGCGCcCGGAc---CGCCGGAu -3' miRNA: 3'- -CGaUCUCGCGaGCCUagacGUGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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