Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10994 | 5' | -54.2 | NC_002794.1 | + | 72879 | 0.73 | 0.723055 |
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Target: 5'- cUCCUCGGccgGGUCGcggcccuCCUCGCCA-CCGu -3' miRNA: 3'- -AGGAGCC---UUAGCau-----GGAGCGGUuGGC- -5' |
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| 10994 | 5' | -54.2 | NC_002794.1 | + | 98989 | 0.73 | 0.713278 |
|
Target: 5'- gCCUCGGuGGUCGgcgGCUUCGUCAGCUu -3' miRNA: 3'- aGGAGCC-UUAGCa--UGGAGCGGUUGGc -5' |
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| 10994 | 5' | -54.2 | NC_002794.1 | + | 143721 | 0.74 | 0.673591 |
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Target: 5'- cCCggCGGGcGUCGU-CCUCGCCcGCCGg -3' miRNA: 3'- aGGa-GCCU-UAGCAuGGAGCGGuUGGC- -5' |
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| 10994 | 5' | -54.2 | NC_002794.1 | + | 73239 | 0.75 | 0.64345 |
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Target: 5'- cUCCUCGGccacuccGUCGUcuccccgaccccACCUcCGCCGACCGc -3' miRNA: 3'- -AGGAGCCu------UAGCA------------UGGA-GCGGUUGGC- -5' |
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| 10994 | 5' | -54.2 | NC_002794.1 | + | 191767 | 0.76 | 0.567196 |
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Target: 5'- cCCUCGGAAUCGU-CCgcgacgcucgaacguUCGCuCGACCGc -3' miRNA: 3'- aGGAGCCUUAGCAuGG---------------AGCG-GUUGGC- -5' |
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| 10994 | 5' | -54.2 | NC_002794.1 | + | 53719 | 0.77 | 0.543509 |
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Target: 5'- cCCUCGGggUggaccCGcGCCaUCGCCGGCCGc -3' miRNA: 3'- aGGAGCCuuA-----GCaUGG-AGCGGUUGGC- -5' |
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| 10994 | 5' | -54.2 | NC_002794.1 | + | 119499 | 0.77 | 0.533734 |
|
Target: 5'- gUCCUCGGggUCGUcggcgGCgCggaGCCGGCCGc -3' miRNA: 3'- -AGGAGCCuuAGCA-----UG-Gag-CGGUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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