miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
110 3' -59.5 AC_000006.1 + 30855 0.66 0.38993
Target:  5'- aCUGGAguaccagaguugGGAgGAGGGuACcgaGGUGCUGCu -3'
miRNA:   3'- gGGCCU------------CCUaCUCCC-UGa--CCGCGACG- -5'
110 3' -59.5 AC_000006.1 + 15540 0.66 0.372207
Target:  5'- -gCGGGGGGUGcAGuGGC-GGCGCgGCg -3'
miRNA:   3'- ggGCCUCCUAC-UCcCUGaCCGCGaCG- -5'
110 3' -59.5 AC_000006.1 + 17654 0.67 0.314608
Target:  5'- gCCUGGcGGcgGgcGGGGACgcGGCGCacgGCg -3'
miRNA:   3'- -GGGCCuCCuaC--UCCCUGa-CCGCGa--CG- -5'
110 3' -59.5 AC_000006.1 + 4780 0.69 0.264008
Target:  5'- uCCCGGAGGA-GGGGGGCcaccuCGUUGa -3'
miRNA:   3'- -GGGCCUCCUaCUCCCUGacc--GCGACg -5'
110 3' -59.5 AC_000006.1 + 10686 0.7 0.203425
Target:  5'- gCCGGGGGGgu-GGGACgcauuUGGCGCaggacgggaUGCa -3'
miRNA:   3'- gGGCCUCCUacuCCCUG-----ACCGCG---------ACG- -5'
110 3' -59.5 AC_000006.1 + 23031 1.13 0.000116
Target:  5'- uCCCGGAGGAUGAGGGACUGGCGCUGCa -3'
miRNA:   3'- -GGGCCUCCUACUCCCUGACCGCGACG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.