Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1101 | 3' | -47.9 | NC_000935.1 | + | 11622 | 0.66 | 0.95773 |
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Target: 5'- uUCAGUGGCUgGGgUugCGUguauccgacuggcGUCGGGg -3' miRNA: 3'- cAGUCACUGAaUUgGugGCA-------------UAGUCC- -5' |
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| 1101 | 3' | -47.9 | NC_000935.1 | + | 34487 | 0.66 | 0.952321 |
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Target: 5'- aUCAGcccaGACUUAACCAUCugucugaaugggcaGUAUCAGu -3' miRNA: 3'- cAGUCa---CUGAAUUGGUGG--------------CAUAGUCc -5' |
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| 1101 | 3' | -47.9 | NC_000935.1 | + | 35424 | 0.7 | 0.799982 |
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Target: 5'- --aAGUGGCUUuggcuuuagcGACCugCGUaugGUCAGGu -3' miRNA: 3'- cagUCACUGAA----------UUGGugGCA---UAGUCC- -5' |
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| 1101 | 3' | -47.9 | NC_000935.1 | + | 8069 | 0.72 | 0.711332 |
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Target: 5'- cUCAGUGACUgguUCGCCaGUuUCAGGc -3' miRNA: 3'- cAGUCACUGAauuGGUGG-CAuAGUCC- -5' |
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| 1101 | 3' | -47.9 | NC_000935.1 | + | 20548 | 1.12 | 0.002339 |
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Target: 5'- gGUCAGUGACUUAACCACCGUAUCAGGa -3' miRNA: 3'- -CAGUCACUGAAUUGGUGGCAUAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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