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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
11026 | 5' | -56.1 | NC_002794.1 | + | 38112 | 0.66 | 0.929651 |
Target: 5'- aGACgcGGGAGAGCcgcgccagcugGGGCgUGGaGUCGAGc -3' miRNA: 3'- -UUGa-CCCUCUCGa----------UCUG-ACC-CAGCUC- -5' |
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11026 | 5' | -56.1 | NC_002794.1 | + | 123685 | 0.68 | 0.85878 |
Target: 5'- gGGCUGGGAGcaGGCgcGACUGGcGggcgUGAGc -3' miRNA: 3'- -UUGACCCUC--UCGauCUGACC-Ca---GCUC- -5' |
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11026 | 5' | -56.1 | NC_002794.1 | + | 132144 | 0.68 | 0.834936 |
Target: 5'- cGCUGGGcccGGGGaucggGGACggucUGGGUCGAGu -3' miRNA: 3'- uUGACCC---UCUCga---UCUG----ACCCAGCUC- -5' |
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11026 | 5' | -56.1 | NC_002794.1 | + | 178058 | 0.69 | 0.809451 |
Target: 5'- aGACUGGaAGAGCgagGGAUccgGGuGUCGAGa -3' miRNA: 3'- -UUGACCcUCUCGa--UCUGa--CC-CAGCUC- -5' |
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11026 | 5' | -56.1 | NC_002794.1 | + | 116049 | 0.72 | 0.604232 |
Target: 5'- gAGCgcagGGGGGAGCgcGACUGGGgucgCGGa -3' miRNA: 3'- -UUGa---CCCUCUCGauCUGACCCa---GCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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