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miRNA: 3'- gAUUGaUCGgCUgC-UCGCUCGGGAGG- -5'
miRNA: 3'- gaUUGa---UCGGCUGCU-CGCuCGGGaGG- -5'
miRNA: 3'- gauUGAU-CGGCUGCU-CG----CUCGGGAGg -5'
miRNA: 3'- gaUUGAUCGGCUGCUCGcUCG-GG----AGG- -5'
miRNA: 3'- -GAUUGAUCGGCUG-----CUCGCU--CGGGa--GG- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 11027 | 5' | -55.4 | NC_002794.1 | + | 109182 | 0.73 | 0.677349 |
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Target: 5'- gUAGC-GGCgGAgGcAGCGGGUCCUCCa -3' miRNA: 3'- gAUUGaUCGgCUgC-UCGCUCGGGAGG- -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 11646 | 0.74 | 0.627156 |
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Target: 5'- -cGACgggcGGCCGACGAcGCG-GCCCgCCg -3' miRNA: 3'- gaUUGa---UCGGCUGCU-CGCuCGGGaGG- -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 92226 | 0.74 | 0.597021 |
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Target: 5'- --cGCUAcGCCGGCGAcGCcaagGAGCCCUCg -3' miRNA: 3'- gauUGAU-CGGCUGCU-CG----CUCGGGAGg -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 22945 | 0.76 | 0.489681 |
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Target: 5'- -cGGCUGGCCGACGGGCcGGCgCCguugUCCc -3' miRNA: 3'- gaUUGAUCGGCUGCUCGcUCG-GG----AGG- -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 189651 | 0.79 | 0.337122 |
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Target: 5'- gCUGACUAGCCGGCuggccGAGCGGucGCCCcggCCg -3' miRNA: 3'- -GAUUGAUCGGCUG-----CUCGCU--CGGGa--GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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