miRNA: 3'- gaUUGAUCGgCUGCuCGCu---CGGG----------AGG- -5'
miRNA: 3'- gauUGauCGG-CUGCUCGCUCg-----GGAGG- -5'
miRNA: 3'- gaUUGaUCGgCUGCUCGCU-----------CGGGaGG- -5'
miRNA: 3'- gaUUGaUCGGCUGCU-CGCuCGGgaGG- -5'
miRNA: 3'- gaUUGauc--GGCUGCU--CGCuCGGGAGG- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11027 | 5' | -55.4 | NC_002794.1 | + | 136760 | 0.66 | 0.959172 |
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Target: 5'- -gAGCUGGCgGACGcGCGccacGCCCagcucgaggaUCCg -3' miRNA: 3'- gaUUGAUCGgCUGCuCGCu---CGGG----------AGG- -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 69588 | 0.66 | 0.959172 |
|
Target: 5'- --cGCgcGUCgGACGAGCGGGaggccgCCUCCa -3' miRNA: 3'- gauUGauCGG-CUGCUCGCUCg-----GGAGG- -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 182348 | 0.66 | 0.962637 |
|
Target: 5'- -gGACgGGCgGGCGGGUGAccgggcgcacgGCCCcCCg -3' miRNA: 3'- gaUUGaUCGgCUGCUCGCU-----------CGGGaGG- -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 76832 | 0.66 | 0.962637 |
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Target: 5'- -cAGC-GGCCGGCGAuGCGcGCCggCCu -3' miRNA: 3'- gaUUGaUCGGCUGCU-CGCuCGGgaGG- -5' |
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| 11027 | 5' | -55.4 | NC_002794.1 | + | 181227 | 0.66 | 0.962637 |
|
Target: 5'- -cGACgagauCCGGCGAcgGCGcGCCUUCCa -3' miRNA: 3'- gaUUGauc--GGCUGCU--CGCuCGGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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