Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11042 | 5' | -55.5 | NC_002794.1 | + | 180374 | 0.68 | 0.861115 |
Target: 5'- ---gGCGCGAGGACGCUGcCGGc--- -3' miRNA: 3'- uucgCGUGCUUCUGCGACaGCCacag -5' |
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11042 | 5' | -55.5 | NC_002794.1 | + | 185175 | 0.67 | 0.889859 |
Target: 5'- cGGCGCggcGCGGucGGCGCgGUCGGcgcgGUCa -3' miRNA: 3'- uUCGCG---UGCUu-CUGCGaCAGCCa---CAG- -5' |
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11042 | 5' | -55.5 | NC_002794.1 | + | 190037 | 0.66 | 0.936371 |
Target: 5'- uAGCGCACGuAG-CGCUGacUCaucGUGUCg -3' miRNA: 3'- uUCGCGUGCuUCuGCGAC--AGc--CACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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