Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11044 | 5' | -56.1 | NC_002794.1 | + | 109181 | 0.69 | 0.821665 |
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Target: 5'- gGUAGcgGCGGAggCaGCgGGUCCUCc -3' miRNA: 3'- aCAUCuaCGCCUa-GaCGaCCGGGAGc -5' |
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| 11044 | 5' | -56.1 | NC_002794.1 | + | 112359 | 0.69 | 0.79555 |
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Target: 5'- cGgcGggGCGGGgagCUGCUGGaaCUCGg -3' miRNA: 3'- aCauCuaCGCCUa--GACGACCggGAGC- -5' |
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| 11044 | 5' | -56.1 | NC_002794.1 | + | 121308 | 0.7 | 0.749253 |
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Target: 5'- ----cGUGCaGAUCggcgGUUGGCCCUCGa -3' miRNA: 3'- acaucUACGcCUAGa---CGACCGGGAGC- -5' |
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| 11044 | 5' | -56.1 | NC_002794.1 | + | 187679 | 0.7 | 0.729967 |
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Target: 5'- gGgcGAUGCGGGUCgaGC-GGCCCgUUGg -3' miRNA: 3'- aCauCUACGCCUAGa-CGaCCGGG-AGC- -5' |
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| 11044 | 5' | -56.1 | NC_002794.1 | + | 118615 | 0.71 | 0.710349 |
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Target: 5'- uUGUAGAUGaugaGGAUgagCUGCUGcGCCagCUCGg -3' miRNA: 3'- -ACAUCUACg---CCUA---GACGAC-CGG--GAGC- -5' |
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| 11044 | 5' | -56.1 | NC_002794.1 | + | 50148 | 0.71 | 0.710349 |
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Target: 5'- --gGGAUGCGGugcaggUUGUUGGUCCUCu -3' miRNA: 3'- acaUCUACGCCua----GACGACCGGGAGc -5' |
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| 11044 | 5' | -56.1 | NC_002794.1 | + | 120733 | 0.71 | 0.68146 |
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Target: 5'- cGUGGGcacgGCGGuccgggagcgcagacUCUGCUGGCCCUg- -3' miRNA: 3'- aCAUCUa---CGCCu--------------AGACGACCGGGAgc -5' |
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| 11044 | 5' | -56.1 | NC_002794.1 | + | 147982 | 0.73 | 0.579507 |
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Target: 5'- gUGUGGcgGUGuccCUGCUGGCCUUCGu -3' miRNA: 3'- -ACAUCuaCGCcuaGACGACCGGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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