Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11048 | 5' | -57.1 | NC_002794.1 | + | 177594 | 0.66 | 0.911292 |
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Target: 5'- -aGGcUCGGCggagCCUcGG-GUCGGUCGa -3' miRNA: 3'- caCCuAGUCGa---GGAaCCuCAGCCGGC- -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 50347 | 0.66 | 0.897861 |
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Target: 5'- -cGGucacCGGCUCCgccaugaccgcGGAGcCGGCCGg -3' miRNA: 3'- caCCua--GUCGAGGaa---------CCUCaGCCGGC- -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 89818 | 0.66 | 0.892709 |
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Target: 5'- uGUGGGgu-GCgUCCUggGGAacgagucgGUCGGCCGg -3' miRNA: 3'- -CACCUaguCG-AGGAa-CCU--------CAGCCGGC- -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 167327 | 0.67 | 0.841895 |
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Target: 5'- cGUGuAUCGGCUUCUUGGAcGUgUGGCUa -3' miRNA: 3'- -CACcUAGUCGAGGAACCU-CA-GCCGGc -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 145586 | 0.67 | 0.841895 |
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Target: 5'- -cGGAgcggcggcCGGCUCCcgcGGAGUCGGgCGc -3' miRNA: 3'- caCCUa-------GUCGAGGaa-CCUCAGCCgGC- -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 118243 | 0.68 | 0.825641 |
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Target: 5'- -aGGAugUCGGCgggcUGGAgGUCGGCCGg -3' miRNA: 3'- caCCU--AGUCGaggaACCU-CAGCCGGC- -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 37754 | 0.68 | 0.825641 |
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Target: 5'- -cGGcgCGGCggcgCCggcgGGAG-CGGCCGu -3' miRNA: 3'- caCCuaGUCGa---GGaa--CCUCaGCCGGC- -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 192804 | 0.68 | 0.808712 |
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Target: 5'- -aGGAUCAcguGCUCCacgUGGAacaGGCCGg -3' miRNA: 3'- caCCUAGU---CGAGGa--ACCUcagCCGGC- -5' |
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| 11048 | 5' | -57.1 | NC_002794.1 | + | 148053 | 0.73 | 0.554091 |
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Target: 5'- -cGGAUCGGCUCCgcgcucugggagccgGGAGU-GGCCu -3' miRNA: 3'- caCCUAGUCGAGGaa-------------CCUCAgCCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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