Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
11053 | 5' | -50.8 | NC_002794.1 | + | 134257 | 0.67 | 0.992817 |
Target: 5'- cGUGUUCa--UCGAcgCGUGCGGcgUGGu -3' miRNA: 3'- -CACAAGgcaAGCUuaGCGCGCCa-ACU- -5' |
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11053 | 5' | -50.8 | NC_002794.1 | + | 146862 | 0.68 | 0.987845 |
Target: 5'- -cGUUCgCGUUCGggUgggggGCGCGGUcGGc -3' miRNA: 3'- caCAAG-GCAAGCuuAg----CGCGCCAaCU- -5' |
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11053 | 5' | -50.8 | NC_002794.1 | + | 90256 | 0.68 | 0.986261 |
Target: 5'- cGUGgucUCCGcgCGAGUgguuuucgcgCGCGCGGUcGAg -3' miRNA: 3'- -CACa--AGGCaaGCUUA----------GCGCGCCAaCU- -5' |
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11053 | 5' | -50.8 | NC_002794.1 | + | 123798 | 0.69 | 0.980553 |
Target: 5'- gGUGgcgCCGUUCGGGg-GCGCGGacGAg -3' miRNA: 3'- -CACaa-GGCAAGCUUagCGCGCCaaCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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