Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11079 | 3' | -55 | NC_002794.1 | + | 36566 | 0.66 | 0.941119 |
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Target: 5'- gCUGCagacacgcgccGCCGAGCAgGCggUCCGcGUGc -3' miRNA: 3'- aGACG-----------UGGUUCGUgCGaaAGGCcCAC- -5' |
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| 11079 | 3' | -55 | NC_002794.1 | + | 76543 | 0.67 | 0.936371 |
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Target: 5'- --gGCGCCGacgcGGuCGCGCg--CCGGGUa -3' miRNA: 3'- agaCGUGGU----UC-GUGCGaaaGGCCCAc -5' |
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| 11079 | 3' | -55 | NC_002794.1 | + | 66826 | 0.67 | 0.936371 |
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Target: 5'- --gGCGCCcGGCGCGac-UCCGGGc- -3' miRNA: 3'- agaCGUGGuUCGUGCgaaAGGCCCac -5' |
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| 11079 | 3' | -55 | NC_002794.1 | + | 12495 | 0.67 | 0.931386 |
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Target: 5'- cCUGCAcguucCCGAGC-CGCUcgaccggcggCUGGGUGg -3' miRNA: 3'- aGACGU-----GGUUCGuGCGAaa--------GGCCCAC- -5' |
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| 11079 | 3' | -55 | NC_002794.1 | + | 108016 | 0.78 | 0.402457 |
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Target: 5'- gCUGCACC-GGCACGUgcucggccuggaaUUCCGGGUGc -3' miRNA: 3'- aGACGUGGuUCGUGCGa------------AAGGCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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