miRNA: 3'- gagUGU-GUUCGAuCCGGGUGCCA--UCG- -5'
miRNA: 3'- gaGUGUGUUCGa-----UC-CGGGUGCCaUCg -5'
miRNA: 3'- -GAGUGUGu--UCGA-----UCCGGGUGCcaUCG- -5'
miRNA: 3'- -GAGUGUGU-----------UCGA--UCCGG--GUGCCa--UCG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11079 | 5' | -54.2 | NC_002794.1 | + | 186873 | 0.71 | 0.787681 |
|
Target: 5'- --aGCAgCGGGCUcGGCUCGCGGUccGGCg -3' miRNA: 3'- gagUGU-GUUCGAuCCGGGUGCCA--UCG- -5' |
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| 11079 | 5' | -54.2 | NC_002794.1 | + | 187501 | 0.71 | 0.805713 |
|
Target: 5'- -cCGCGCAGGCggccagGGcGCCCGCGGaGGa -3' miRNA: 3'- gaGUGUGUUCGa-----UC-CGGGUGCCaUCg -5' |
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| 11079 | 5' | -54.2 | NC_002794.1 | + | 190590 | 0.66 | 0.971928 |
|
Target: 5'- uCUCGCGCcgcAGCUccuccAGGUCCGCGacAGCc -3' miRNA: 3'- -GAGUGUGu--UCGA-----UCCGGGUGCcaUCG- -5' |
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| 11079 | 5' | -54.2 | NC_002794.1 | + | 193048 | 0.7 | 0.831558 |
|
Target: 5'- aCUCGCACAgcgcccgcaccGGCUgcGGGCCguCGCGGgacAGCc -3' miRNA: 3'- -GAGUGUGU-----------UCGA--UCCGG--GUGCCa--UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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