Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11086 | 5' | -45.3 | NC_002794.1 | + | 19838 | 0.66 | 0.999999 |
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Target: 5'- uGCUGCAcgGc-UACGGGGaCUGCGu -3' miRNA: 3'- cUGACGUuaUaaAUGUUCCaGACGCc -5' |
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| 11086 | 5' | -45.3 | NC_002794.1 | + | 118881 | 0.66 | 0.999998 |
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Target: 5'- aGCUGCAggucggcccgGUGUUcGCGuAGGUCcgugGCGGu -3' miRNA: 3'- cUGACGU----------UAUAAaUGU-UCCAGa---CGCC- -5' |
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| 11086 | 5' | -45.3 | NC_002794.1 | + | 140161 | 0.68 | 0.999965 |
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Target: 5'- ---cGCAGUAUUUACGauuuuggaucGGGUCgGCGa -3' miRNA: 3'- cugaCGUUAUAAAUGU----------UCCAGaCGCc -5' |
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| 11086 | 5' | -45.3 | NC_002794.1 | + | 117422 | 0.69 | 0.999912 |
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Target: 5'- cGCUGCAGgagcugccGCGAGGcgUUGCGGa -3' miRNA: 3'- cUGACGUUauaaa---UGUUCCa-GACGCC- -5' |
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| 11086 | 5' | -45.3 | NC_002794.1 | + | 119492 | 0.69 | 0.99989 |
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Target: 5'- cGGCUGCGuccu---CGGGGUCgucgGCGGc -3' miRNA: 3'- -CUGACGUuauaaauGUUCCAGa---CGCC- -5' |
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| 11086 | 5' | -45.3 | NC_002794.1 | + | 120876 | 0.69 | 0.999856 |
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Target: 5'- cGGCUGguGUccgaacgGCGAGGUCaccGCGGg -3' miRNA: 3'- -CUGACguUAuaaa---UGUUCCAGa--CGCC- -5' |
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| 11086 | 5' | -45.3 | NC_002794.1 | + | 86969 | 0.7 | 0.999664 |
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Target: 5'- cGCUGCGGcgagcugcgACGAGGUCcgGCGGc -3' miRNA: 3'- cUGACGUUauaaa----UGUUCCAGa-CGCC- -5' |
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| 11086 | 5' | -45.3 | NC_002794.1 | + | 126237 | 0.7 | 0.999514 |
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Target: 5'- gGACcgugGCGAUcgUggcgcGCGAGGUCgGCGGc -3' miRNA: 3'- -CUGa---CGUUAuaAa----UGUUCCAGaCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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