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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11091 | 5' | -55.4 | NC_002794.1 | + | 181774 | 0.66 | 0.965488 |
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Target: 5'- -aUGGcGCCGCGccgccGcGUCUCgUUCGGGCCu -3' miRNA: 3'- gaGCC-UGGUGCu----C-CAGAG-AAGCCUGG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 21726 | 0.66 | 0.965488 |
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Target: 5'- gUCGcGAUCGCGAGGcCgaagCGGugCg -3' miRNA: 3'- gAGC-CUGGUGCUCCaGagaaGCCugG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 102541 | 0.66 | 0.965488 |
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Target: 5'- uCUCGGGCCGCGuGGagaCggCGGcCCu -3' miRNA: 3'- -GAGCCUGGUGCuCCagaGaaGCCuGG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 146942 | 0.66 | 0.965488 |
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Target: 5'- gCUCGGuCUgGCGuGGcucUCUCUUCauGGACCg -3' miRNA: 3'- -GAGCCuGG-UGCuCC---AGAGAAG--CCUGG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 149495 | 0.66 | 0.965488 |
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Target: 5'- gCUCGGGgauggggauggUgACGGGGUCgUCgccgggagCGGACCu -3' miRNA: 3'- -GAGCCU-----------GgUGCUCCAG-AGaa------GCCUGG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 124928 | 0.66 | 0.968526 |
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Target: 5'- cCUCGGugCGCGuGGaccucgacuUCUCcgUGGugCu -3' miRNA: 3'- -GAGCCugGUGCuCC---------AGAGaaGCCugG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 80755 | 0.66 | 0.968526 |
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Target: 5'- -cCGGGCCACGcgcucGGacCUCUUC-GACCu -3' miRNA: 3'- gaGCCUGGUGCu----CCa-GAGAAGcCUGG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 112293 | 0.66 | 0.968526 |
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Target: 5'- gUCGGGCgGCcccccGGGUCcgaCUUCGGcGCCg -3' miRNA: 3'- gAGCCUGgUGc----UCCAGa--GAAGCC-UGG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 43674 | 0.66 | 0.968526 |
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Target: 5'- uCUCGGGCC-CG-GG-Cg---CGGACCa -3' miRNA: 3'- -GAGCCUGGuGCuCCaGagaaGCCUGG- -5' |
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| 11091 | 5' | -55.4 | NC_002794.1 | + | 122000 | 0.66 | 0.971093 |
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Target: 5'- -cCGGGagcuguuCCACGucGUCuUCUUCGGcGCCg -3' miRNA: 3'- gaGCCU-------GGUGCucCAG-AGAAGCC-UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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