Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11098 | 3' | -55.3 | NC_002794.1 | + | 145194 | 0.66 | 0.959868 |
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Target: 5'- gGUCGC-CGCGGCggCccUCCGGCaGCGc -3' miRNA: 3'- gCAGUGcGCGCUGaaGa-AGGUCG-CGU- -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 147004 | 0.66 | 0.943934 |
|
Target: 5'- aCGUCgGCG-GCGGCggCcgCCGGCGCu -3' miRNA: 3'- -GCAG-UGCgCGCUGaaGaaGGUCGCGu -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 187941 | 0.67 | 0.913162 |
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Target: 5'- gCGUCACGCGUGcGCgcg--CgAGCGCGg -3' miRNA: 3'- -GCAGUGCGCGC-UGaagaaGgUCGCGU- -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 188039 | 0.69 | 0.843878 |
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Target: 5'- cCGUCccggACGCGgcgagucuCGACUUCggCCAGCGUc -3' miRNA: 3'- -GCAG----UGCGC--------GCUGAAGaaGGUCGCGu -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 192413 | 0.68 | 0.894658 |
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Target: 5'- aGUCgaaACGcCGCGACgggUCcgcggCCGGCGCGu -3' miRNA: 3'- gCAG---UGC-GCGCUGa--AGaa---GGUCGCGU- -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 193816 | 0.69 | 0.866882 |
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Target: 5'- uCGUCugGCGcCGACgcg-UCgCGGCGCc -3' miRNA: 3'- -GCAGugCGC-GCUGaagaAG-GUCGCGu -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 194283 | 0.68 | 0.881201 |
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Target: 5'- aCGgCGCGCGCcACguccagUCgUCCAGCGCc -3' miRNA: 3'- -GCaGUGCGCGcUGa-----AGaAGGUCGCGu -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 194784 | 0.67 | 0.913162 |
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Target: 5'- cCGgCACGCGCGcaGCggCUcCCAGUGCc -3' miRNA: 3'- -GCaGUGCGCGC--UGaaGAaGGUCGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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