Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11098 | 3' | -55.3 | NC_002794.1 | + | 44574 | 0.67 | 0.91943 |
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Target: 5'- aCGUCACGCGgGACcgagccaccgccgcCUUCUugGGCGCc -3' miRNA: 3'- -GCAGUGCGCgCUGaa------------GAAGG--UCGCGu -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 129054 | 0.67 | 0.918871 |
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Target: 5'- uCGUgGCGCGCGACggugugUCgcggCUGGCgGCGg -3' miRNA: 3'- -GCAgUGCGCGCUGa-----AGaa--GGUCG-CGU- -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 58556 | 0.67 | 0.92273 |
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Target: 5'- gCGcugCugGCGCGcugcgcgaacgccuACUUCcaCCAGCGCGg -3' miRNA: 3'- -GCa--GugCGCGC--------------UGAAGaaGGUCGCGU- -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 138138 | 0.67 | 0.934605 |
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Target: 5'- gGUCuCGgGCGGCggUUUCUuGCGCAg -3' miRNA: 3'- gCAGuGCgCGCUGaaGAAGGuCGCGU- -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 143188 | 0.67 | 0.934605 |
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Target: 5'- gGUUcucgACGUGCaGCUUCUcgcggucCCAGCGCAg -3' miRNA: 3'- gCAG----UGCGCGcUGAAGAa------GGUCGCGU- -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 82617 | 0.67 | 0.939385 |
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Target: 5'- aGUCACggaacuGCGCGaACUcaUCcgCCGGCGCc -3' miRNA: 3'- gCAGUG------CGCGC-UGA--AGaaGGUCGCGu -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 50571 | 0.67 | 0.924349 |
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Target: 5'- uGUCGacacggcgUGCGCGGCggCUggguggCCGGCGCc -3' miRNA: 3'- gCAGU--------GCGCGCUGaaGAa-----GGUCGCGu -5' |
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| 11098 | 3' | -55.3 | NC_002794.1 | + | 44710 | 0.66 | 0.963304 |
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Target: 5'- gGcCGCGacCGCGGCgacgUCggCCGGCGCc -3' miRNA: 3'- gCaGUGC--GCGCUGa---AGaaGGUCGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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