Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 111 | 5' | -56.7 | AC_000006.1 | + | 20794 | 0.66 | 0.500121 |
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Target: 5'- aCGCC-UCCGGuCUCgcgGCCGGccguguugacaauGGCGCa -3' miRNA: 3'- aGCGGaAGGUU-GAGa--UGGUC-------------CCGCGa -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 11637 | 0.66 | 0.479983 |
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Target: 5'- cUCGCCg-CCGGCggcgccccCUACCAGcgcccGGCGCa -3' miRNA: 3'- -AGCGGaaGGUUGa-------GAUGGUC-----CCGCGa -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 25695 | 0.66 | 0.469544 |
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Target: 5'- aCGCgggUUgAGCUUUGCCuGGGCGUUg -3' miRNA: 3'- aGCGga-AGgUUGAGAUGGuCCCGCGA- -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 3804 | 0.66 | 0.459223 |
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Target: 5'- -gGgCUUCCAGCUCgGCCAgcaGGGCcaGCa -3' miRNA: 3'- agCgGAAGGUUGAGaUGGU---CCCG--CGa -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 16610 | 0.67 | 0.419199 |
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Target: 5'- gCGCCgcguaaCCAACgc-GCCGGGGcCGCUc -3' miRNA: 3'- aGCGGaa----GGUUGagaUGGUCCC-GCGA- -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 1805 | 0.68 | 0.389677 |
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Target: 5'- -aGCCUugauuuuUCCAGC----CCAGGGCGCa -3' miRNA: 3'- agCGGA-------AGGUUGagauGGUCCCGCGa -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 8474 | 0.69 | 0.337383 |
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Target: 5'- gUCGUCgcgcacgCCAGCgCUcUCAGGGCGCa -3' miRNA: 3'- -AGCGGaa-----GGUUGaGAuGGUCCCGCGa -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 24144 | 0.72 | 0.199076 |
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Target: 5'- cCGCCUUUCAGCgUCUGCCucaGCGCUc -3' miRNA: 3'- aGCGGAAGGUUG-AGAUGGuccCGCGA- -5' |
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| 111 | 5' | -56.7 | AC_000006.1 | + | 23442 | 1.08 | 0.000384 |
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Target: 5'- gUCGCCUUCCAACUCUACCAGGGCGCUa -3' miRNA: 3'- -AGCGGAAGGUUGAGAUGGUCCCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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