miRNA: 3'- -CUAGCaa----G-CUGGGCCAUaaCGCCG-GC- -5'
miRNA: 3'- cUAGCaAGCuGGGCCauaa-CGCCGGC- -5'
miRNA: 3'- -CUAGCa--AGCUG-GGCCauaaCGCCGGC- -5'
miRNA: 3'- cuaGCAAGC--UGGGCCauaaCGCCGGC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11107 | 3' | -54.8 | NC_002794.1 | + | 36993 | 0.73 | 0.687312 |
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Target: 5'- aGGUCGgcgaggCgGACCCGGUAgaGCGGCuCGg -3' miRNA: 3'- -CUAGCaa----G-CUGGGCCAUaaCGCCG-GC- -5' |
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| 11107 | 3' | -54.8 | NC_002794.1 | + | 154330 | 0.74 | 0.667349 |
|
Target: 5'- cGUCGgUCGuCCCGGgugccGCGGCCGa -3' miRNA: 3'- cUAGCaAGCuGGGCCauaa-CGCCGGC- -5' |
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| 11107 | 3' | -54.8 | NC_002794.1 | + | 121201 | 0.74 | 0.637218 |
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Target: 5'- cGAUCGgggUCGACuUCGGgg--GCGGCCGc -3' miRNA: 3'- -CUAGCa--AGCUG-GGCCauaaCGCCGGC- -5' |
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| 11107 | 3' | -54.8 | NC_002794.1 | + | 50723 | 0.76 | 0.557218 |
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Target: 5'- --gCGUUCGccGCCCGGg---GCGGCCGc -3' miRNA: 3'- cuaGCAAGC--UGGGCCauaaCGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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