miRNA: 3'- -CUGGggCGCUGGCUUa---------GCCGCUc--ACC- -5'
miRNA: 3'- cUGGgGCGCUG--GCUuaGCCGCUCaCc -5'
miRNA: 3'- cugGGGCGCUGGcuuAGCCGCUCaCc -5'
miRNA: 3'- cUG-GGGCGCUGGCuua---GCCGCUca-CC- -5'
miRNA: 3'- cUGGGGC-GCUGGC--------UUAgCCGCUCa-CC- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11114 | 5' | -58.4 | NC_002794.1 | + | 139004 | 0.66 | 0.903849 |
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Target: 5'- aGGCUuuGCGGCUGAAgagcucgccgCGGCGAaacUGGc -3' miRNA: 3'- -CUGGggCGCUGGCUUa---------GCCGCUc--ACC- -5' |
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| 11114 | 5' | -58.4 | NC_002794.1 | + | 53264 | 0.66 | 0.903849 |
|
Target: 5'- cGCCgCGCGACaaCGGggCGGCGAGcGa -3' miRNA: 3'- cUGGgGCGCUG--GCUuaGCCGCUCaCc -5' |
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| 11114 | 5' | -58.4 | NC_002794.1 | + | 193742 | 0.66 | 0.903849 |
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Target: 5'- --gCCCGCGGCU---UCGGCGAGcGa -3' miRNA: 3'- cugGGGCGCUGGcuuAGCCGCUCaCc -5' |
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| 11114 | 5' | -58.4 | NC_002794.1 | + | 60465 | 0.66 | 0.903849 |
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Target: 5'- cGCuCCCGCGACUGcauccgCGGCGAcacGGa -3' miRNA: 3'- cUG-GGGCGCUGGCuua---GCCGCUca-CC- -5' |
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| 11114 | 5' | -58.4 | NC_002794.1 | + | 48556 | 0.66 | 0.903849 |
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Target: 5'- cACUCCGcCGGCCGcccguccaGAUgGGCGAGccGGa -3' miRNA: 3'- cUGGGGC-GCUGGC--------UUAgCCGCUCa-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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