Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1112 | 3' | -57.6 | NC_000940.1 | + | 2478 | 0.66 | 0.139008 |
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Target: 5'- --cCCACCCcagagGUGAucaauuugacgaugcCAGGGaUGGUGCa -3' miRNA: 3'- guuGGUGGGca---CACU---------------GUCCC-ACCGCG- -5' |
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| 1112 | 3' | -57.6 | NC_000940.1 | + | 3458 | 0.66 | 0.137251 |
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Target: 5'- -cGCCACCC-UGUcACAcGGGUGGuUGUg -3' miRNA: 3'- guUGGUGGGcACAcUGU-CCCACC-GCG- -5' |
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| 1112 | 3' | -57.6 | NC_000940.1 | + | 4467 | 0.69 | 0.076555 |
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Target: 5'- cCAACCugCCcacggcugGUGAgcaCAGGGUGGCu- -3' miRNA: 3'- -GUUGGugGGca------CACU---GUCCCACCGcg -5' |
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| 1112 | 3' | -57.6 | NC_000940.1 | + | 150 | 0.7 | 0.060728 |
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Target: 5'- uCGACC-CCCGgacguguuUGUGGCgcaaGGGGUGGUGg -3' miRNA: 3'- -GUUGGuGGGC--------ACACUG----UCCCACCGCg -5' |
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| 1112 | 3' | -57.6 | NC_000940.1 | + | 3427 | 1.12 | 0.000016 |
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Target: 5'- aCAACCACCCGUGUGACAGGGUGGCGCg -3' miRNA: 3'- -GUUGGUGGGCACACUGUCCCACCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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