Results 21 - 22 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11127 | 3' | -58.2 | NC_002794.1 | + | 22361 | 0.72 | 0.54886 |
Target: 5'- -cCUCUC-CCGCUcaCCCCGguccCGAUCCGg -3' miRNA: 3'- aaGAGAGaGGCGAa-GGGGC----GCUAGGC- -5' |
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11127 | 3' | -58.2 | NC_002794.1 | + | 44035 | 0.75 | 0.37088 |
Target: 5'- gUCgCUCUCCGC--CCCCGgGGUCCGc -3' miRNA: 3'- aAGaGAGAGGCGaaGGGGCgCUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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