Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11131 | 3' | -53.1 | NC_002794.1 | + | 80409 | 0.67 | 0.981748 |
Target: 5'- --cCGCGgccCAGGUGGcgccGGGCGG-GGc -3' miRNA: 3'- guuGUGCaa-GUCCACCu---UCCGCCaCC- -5' |
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11131 | 3' | -53.1 | NC_002794.1 | + | 179967 | 0.67 | 0.974883 |
Target: 5'- aCAGCAgGagCGGcaGGAgccaAGGCGGUGGc -3' miRNA: 3'- -GUUGUgCaaGUCcaCCU----UCCGCCACC- -5' |
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11131 | 3' | -53.1 | NC_002794.1 | + | 126709 | 0.68 | 0.963032 |
Target: 5'- --cCGCGgacUCGGGgcgaGGggGGUgGGUGGg -3' miRNA: 3'- guuGUGCa--AGUCCa---CCuuCCG-CCACC- -5' |
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11131 | 3' | -53.1 | NC_002794.1 | + | 123406 | 0.67 | 0.974105 |
Target: 5'- --cCGCG-UCGGcGUGGAguacgacagcgacgAGGCGGUGa -3' miRNA: 3'- guuGUGCaAGUC-CACCU--------------UCCGCCACc -5' |
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11131 | 3' | -53.1 | NC_002794.1 | + | 135648 | 0.69 | 0.942913 |
Target: 5'- aCAGCGCGga-GGGUGGggGGguguccuccacgaCGGgcagGGg -3' miRNA: 3'- -GUUGUGCaagUCCACCuuCC-------------GCCa---CC- -5' |
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11131 | 3' | -53.1 | NC_002794.1 | + | 146865 | 0.8 | 0.419948 |
Target: 5'- --uCGCGUUCGGGUGGggGGCgcGGUcGGc -3' miRNA: 3'- guuGUGCAAGUCCACCuuCCG--CCA-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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