Results 61 - 62 of 62 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11133 | 5' | -57.1 | NC_002794.1 | + | 6131 | 0.66 | 0.916145 |
Target: 5'- gUUGCGGUcgaucgGGGGGCgGAGcCCGA--GCa -3' miRNA: 3'- -AGCGCCA------UCUCCGgCUCuGGCUacUG- -5' |
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11133 | 5' | -57.1 | NC_002794.1 | + | 4177 | 0.67 | 0.885269 |
Target: 5'- gUCGCGGUGGGucGUCGAGGCaaggCGAugcugUGACg -3' miRNA: 3'- -AGCGCCAUCUc-CGGCUCUG----GCU-----ACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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