miRNA: 3'- -GGAC-UGUAAGa--UCG-----CUGGUCGgCG-CC- -5'
miRNA: 3'- -GgACUGUaAGa-------UCGCUGG--UC-GGCGCC- -5'
miRNA: 3'- -GGACug-UAAGauc-GCUGGUCGGCGcc -5'
miRNA: 3'- -GGaCUGUAaga-UCGCUGGucGGCGCC- -5'
miRNA: 3'- -GGaCUGuAAGAUcgCUGGUCGGCG-CC- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11138 | 3' | -54.2 | NC_002794.1 | + | 12494 | 0.69 | 0.917011 |
|
Target: 5'- aCCUGcACGUUCccgAGCcgcucGACCGGCgGCuGGg -3' miRNA: 3'- -GGAC-UGUAAGa--UCG-----CUGGUCGgCG-CC- -5' |
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| 11138 | 3' | -54.2 | NC_002794.1 | + | 11519 | 0.68 | 0.937777 |
|
Target: 5'- aCgUGACGaUCgagcugcgGGCGACCgaGGaCCGCGGc -3' miRNA: 3'- -GgACUGUaAGa-------UCGCUGG--UC-GGCGCC- -5' |
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| 11138 | 3' | -54.2 | NC_002794.1 | + | 8865 | 0.66 | 0.982988 |
|
Target: 5'- cCCUGcgaGUUCcucaCGGCCAGCCGCc- -3' miRNA: 3'- -GGACug-UAAGauc-GCUGGUCGGCGcc -5' |
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| 11138 | 3' | -54.2 | NC_002794.1 | + | 4045 | 0.66 | 0.98101 |
|
Target: 5'- aCC-GGCAUcaugAGCGACCc-CCGCGGc -3' miRNA: 3'- -GGaCUGUAaga-UCGCUGGucGGCGCC- -5' |
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| 11138 | 3' | -54.2 | NC_002794.1 | + | 1281 | 0.66 | 0.974044 |
|
Target: 5'- aCC-GGCcUUCUAagGACCGGCCGCuGGc -3' miRNA: 3'- -GGaCUGuAAGAUcgCUGGUCGGCG-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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