Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11143 | 3' | -55.3 | NC_002794.1 | + | 147143 | 0.66 | 0.947944 |
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Target: 5'- cGAgaCGGCCCCGGGgccgcgagGGCCagcUGGAc -3' miRNA: 3'- -CUgaGUCGGGGCCCa-------CUGGaa-ACUUu -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 151661 | 0.66 | 0.947944 |
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Target: 5'- uGGCUC-GCCgCCGGGUGACgaccGAGGg -3' miRNA: 3'- -CUGAGuCGG-GGCCCACUGgaaaCUUU- -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 19776 | 0.66 | 0.943527 |
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Target: 5'- gGAC-CAGCUCCGGGUGcuCCUg----- -3' miRNA: 3'- -CUGaGUCGGGGCCCACu-GGAaacuuu -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 108030 | 0.66 | 0.928316 |
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Target: 5'- uGCUCGGCCuggaauuCCGGGUG-CCguucgUGAAc -3' miRNA: 3'- cUGAGUCGG-------GGCCCACuGGaa---ACUUu -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 105413 | 0.66 | 0.923466 |
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Target: 5'- gGAuCUCGGCCuCCaGGGUGGCCg------ -3' miRNA: 3'- -CU-GAGUCGG-GG-CCCACUGGaaacuuu -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 151956 | 0.67 | 0.892994 |
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Target: 5'- cGACgaCGGCCCCGGGUGcuCCUc----- -3' miRNA: 3'- -CUGa-GUCGGGGCCCACu-GGAaacuuu -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 131320 | 0.67 | 0.886196 |
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Target: 5'- -cCUC-GCCCUGGGUGGCCg------ -3' miRNA: 3'- cuGAGuCGGGGCCCACUGGaaacuuu -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 44263 | 0.67 | 0.886196 |
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Target: 5'- cGGCUCGGCCgCCGGG-GACg---GAGGa -3' miRNA: 3'- -CUGAGUCGG-GGCCCaCUGgaaaCUUU- -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 126449 | 0.71 | 0.731302 |
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Target: 5'- -cCUCGGCCCUGGGUccggcucgucggGACCUUggccUGggGa -3' miRNA: 3'- cuGAGUCGGGGCCCA------------CUGGAA----ACuuU- -5' |
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| 11143 | 3' | -55.3 | NC_002794.1 | + | 178802 | 0.72 | 0.650371 |
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Target: 5'- cGGCUCGGCCCgGGGgGACCg--GGc- -3' miRNA: 3'- -CUGAGUCGGGgCCCaCUGGaaaCUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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