Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11147 | 3' | -53.3 | NC_002794.1 | + | 43960 | 0.66 | 0.98918 |
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Target: 5'- gUCCUGGUcCGUCCag-AUGAgcuGGUCGu -3' miRNA: 3'- gAGGGCCA-GCAGGgaaUAUU---CCAGCc -5' |
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| 11147 | 3' | -53.3 | NC_002794.1 | + | 142903 | 0.67 | 0.980688 |
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Target: 5'- -gCCCGG-CGUCCUccg-GGGGcCGGa -3' miRNA: 3'- gaGGGCCaGCAGGGaauaUUCCaGCC- -5' |
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| 11147 | 3' | -53.3 | NC_002794.1 | + | 179106 | 0.67 | 0.980478 |
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Target: 5'- -gUCCGGUCGUCCgCgguccgggcgcgcGGUCGGu -3' miRNA: 3'- gaGGGCCAGCAGG-Gaauauu-------CCAGCC- -5' |
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| 11147 | 3' | -53.3 | NC_002794.1 | + | 111024 | 0.67 | 0.977126 |
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Target: 5'- gCUCCCGGUCGUCgUgauccggcuGG-CGGu -3' miRNA: 3'- -GAGGGCCAGCAGgGaauauu---CCaGCC- -5' |
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| 11147 | 3' | -53.3 | NC_002794.1 | + | 47281 | 0.67 | 0.976165 |
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Target: 5'- uUCgCGGUCuuUCCCUcgccccGAGGUCGGu -3' miRNA: 3'- gAGgGCCAGc-AGGGAaua---UUCCAGCC- -5' |
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| 11147 | 3' | -53.3 | NC_002794.1 | + | 52872 | 0.69 | 0.946078 |
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Target: 5'- cCUUCCGGUacaCCCgaugGUGGGGUCGa -3' miRNA: 3'- -GAGGGCCAgcaGGGaa--UAUUCCAGCc -5' |
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| 11147 | 3' | -53.3 | NC_002794.1 | + | 89850 | 0.69 | 0.941651 |
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Target: 5'- --gCCGGcCGUCCCUUucucGGUUGGc -3' miRNA: 3'- gagGGCCaGCAGGGAAuauuCCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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