miRNA: 3'- -ACUGGUU--AGUAUUaaUUUUGUCGgag -5'
miRNA: 3'- -ACUGGUUAGUAUUAAUuuuGUCGGAg -5'
miRNA: 3'- -ACUGGUUaGUAUuaauuuUGUCGGag -5'
miRNA: 3'- -ACUGGUUAGuauUAAUuuUGUCGGAg -5'
miRNA: 3'- -ACUGGUUAGUAUUAAUUUUGUCGGAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11154 | 5' | -41.4 | NC_002796.1 | + | 1303 | 0.67 | 0.999697 |
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Target: 5'- uUGACCAGcuUCAUGA--GAAAUAGCa-- -3' miRNA: 3'- -ACUGGUU--AGUAUUaaUUUUGUCGgag -5' |
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| 11154 | 5' | -41.4 | NC_002796.1 | + | 23517 | 0.68 | 0.99933 |
|
Target: 5'- uUGGCUGAUUAUAGUUGu--CGGUCUg -3' miRNA: 3'- -ACUGGUUAGUAUUAAUuuuGUCGGAg -5' |
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| 11154 | 5' | -41.4 | NC_002796.1 | + | 6146 | 0.68 | 0.998627 |
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Target: 5'- cUGACCAAcCAUGugaucuACAGCCg- -3' miRNA: 3'- -ACUGGUUaGUAUuaauuuUGUCGGag -5' |
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| 11154 | 5' | -41.4 | NC_002796.1 | + | 4293 | 0.73 | 0.959216 |
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Target: 5'- uUGACCAAUCu--AUUGcuGCAGCUUa -3' miRNA: 3'- -ACUGGUUAGuauUAAUuuUGUCGGAg -5' |
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| 11154 | 5' | -41.4 | NC_002796.1 | + | 11413 | 1.13 | 0.011649 |
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Target: 5'- uUGACCAAUCAUAAUUAAAACAGCCUCu -3' miRNA: 3'- -ACUGGUUAGUAUUAAUUUUGUCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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