Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11176 | 3' | -53.2 | NC_002816.1 | + | 37823 | 1.09 | 0.003641 |
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Target: 5'- aGCGCCCACCGACAAUGGCACUUACUAu -3' miRNA: 3'- -CGCGGGUGGCUGUUACCGUGAAUGAU- -5' |
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| 11176 | 3' | -53.2 | NC_002816.1 | + | 7348 | 0.72 | 0.681951 |
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Target: 5'- gGCGCCCGCUGAUAc-GGCACaUUAUUc -3' miRNA: 3'- -CGCGGGUGGCUGUuaCCGUG-AAUGAu -5' |
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| 11176 | 3' | -53.2 | NC_002816.1 | + | 113986 | 0.68 | 0.855145 |
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Target: 5'- uUGCCCACCGGCAAUG--ACUUGa-- -3' miRNA: 3'- cGCGGGUGGCUGUUACcgUGAAUgau -5' |
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| 11176 | 3' | -53.2 | NC_002816.1 | + | 88311 | 0.68 | 0.863161 |
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Target: 5'- gGCGCUCACCGAagcAUGGUucaucauUUUGCUGg -3' miRNA: 3'- -CGCGGGUGGCUgu-UACCGu------GAAUGAU- -5' |
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| 11176 | 3' | -53.2 | NC_002816.1 | + | 34222 | 0.67 | 0.912599 |
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Target: 5'- aGCGCUaCAUCGACAGUGuGCucgACUcGCUGu -3' miRNA: 3'- -CGCGG-GUGGCUGUUAC-CG---UGAaUGAU- -5' |
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| 11176 | 3' | -53.2 | NC_002816.1 | + | 64897 | 0.67 | 0.912599 |
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Target: 5'- cGCGCCCGCCcGCGAcaUGaCAUUUGCc- -3' miRNA: 3'- -CGCGGGUGGcUGUU--ACcGUGAAUGau -5' |
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| 11176 | 3' | -53.2 | NC_002816.1 | + | 84323 | 0.66 | 0.935264 |
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Target: 5'- uCGCaCACCGACAucGGCACacACUGc -3' miRNA: 3'- cGCGgGUGGCUGUuaCCGUGaaUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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