Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11178 | 3' | -51.8 | NC_002816.1 | + | 49331 | 1.09 | 0.006189 |
Target: 5'- aAGCCACCGUGAAAACGGUCAAAGCCGc -3' miRNA: 3'- -UCGGUGGCACUUUUGCCAGUUUCGGC- -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 60313 | 0.72 | 0.78547 |
Target: 5'- aGGCCGCCGUGucguGACGGauuUCugcccGCCGa -3' miRNA: 3'- -UCGGUGGCACuu--UUGCC---AGuuu--CGGC- -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 29492 | 0.71 | 0.822494 |
Target: 5'- uGGCCACgCGU-AAAGUGGUCGucGCCGa -3' miRNA: 3'- -UCGGUG-GCAcUUUUGCCAGUuuCGGC- -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 13720 | 0.67 | 0.950228 |
Target: 5'- cGCCACCacGUGAccgcACGGUaCGAAGCa- -3' miRNA: 3'- uCGGUGG--CACUuu--UGCCA-GUUUCGgc -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 60208 | 0.67 | 0.954436 |
Target: 5'- uGUCugUGUGAAAAUGGUUuuguGCCc -3' miRNA: 3'- uCGGugGCACUUUUGCCAGuuu-CGGc -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 93965 | 0.67 | 0.954436 |
Target: 5'- gAGcCCGCCGUGAAAGCcaUCAAAuuGUCGc -3' miRNA: 3'- -UC-GGUGGCACUUUUGccAGUUU--CGGC- -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 71270 | 0.67 | 0.962115 |
Target: 5'- gAGUaCAUgGUGAAGACGGagAcgauAAGCCGg -3' miRNA: 3'- -UCG-GUGgCACUUUUGCCagU----UUCGGC- -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 32013 | 0.67 | 0.965595 |
Target: 5'- cGGCCAUCGUGAAAacaaacaacACGGgc---GCCa -3' miRNA: 3'- -UCGGUGGCACUUU---------UGCCaguuuCGGc -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 54317 | 0.66 | 0.977274 |
Target: 5'- cGCCGCCGUGucagcccacaGAGACGaaGUCAcAGCa- -3' miRNA: 3'- uCGGUGGCAC----------UUUUGC--CAGUuUCGgc -5' |
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11178 | 3' | -51.8 | NC_002816.1 | + | 122204 | 0.66 | 0.981873 |
Target: 5'- uGCgGCgGUGggGAgGGUCAaacaacacGAGUCa -3' miRNA: 3'- uCGgUGgCACuuUUgCCAGU--------UUCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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