Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11179 | 5' | -50.2 | NC_002816.1 | + | 77629 | 0.66 | 0.991944 |
Target: 5'- uCGUGCAcuGa-GCCACCAgaacaaucguccggUGCACGCc -3' miRNA: 3'- uGUAUGUuuCgcCGGUGGU--------------AUGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 9572 | 0.66 | 0.99111 |
Target: 5'- cACAUACAccucaAAGUaGCCAgguUCGUGCACGg -3' miRNA: 3'- -UGUAUGU-----UUCGcCGGU---GGUAUGUGCg -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 98989 | 0.66 | 0.988946 |
Target: 5'- -gGUGCcAAGUGcGCCAUCGcccgacgaccaccacUACGCGCu -3' miRNA: 3'- ugUAUGuUUCGC-CGGUGGU---------------AUGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 67995 | 0.66 | 0.986735 |
Target: 5'- cCAUACGGuugaugucgcAGuUGGCCuCUAUGCugGCg -3' miRNA: 3'- uGUAUGUU----------UC-GCCGGuGGUAUGugCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 55760 | 0.66 | 0.986735 |
Target: 5'- gACA-ACAAGGCGGUgAUUuUGCAgGCg -3' miRNA: 3'- -UGUaUGUUUCGCCGgUGGuAUGUgCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 36664 | 0.66 | 0.986735 |
Target: 5'- --uUugAGGGCGuucauuGCCACCuuGUACACGUc -3' miRNA: 3'- uguAugUUUCGC------CGGUGG--UAUGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 108573 | 0.67 | 0.983006 |
Target: 5'- cUAUGgauGGGCGGUgACCAUACauaaaGCGCa -3' miRNA: 3'- uGUAUgu-UUCGCCGgUGGUAUG-----UGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 87548 | 0.67 | 0.975998 |
Target: 5'- cGCAUGCA----GGCCACUAucaauuauUAUACGCg -3' miRNA: 3'- -UGUAUGUuucgCCGGUGGU--------AUGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 116577 | 0.68 | 0.970278 |
Target: 5'- cACGUAuuauCAAcucGGCGGCCGCUuUACACu- -3' miRNA: 3'- -UGUAU----GUU---UCGCCGGUGGuAUGUGcg -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 98118 | 0.69 | 0.956036 |
Target: 5'- ---aGgGAGGCGGCCACCAcaaaAUugGUc -3' miRNA: 3'- uguaUgUUUCGCCGGUGGUa---UGugCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 101457 | 0.69 | 0.946955 |
Target: 5'- gGCGUAUGAAccgaacucggcacGCGGCUGaauaUAUACACGCg -3' miRNA: 3'- -UGUAUGUUU-------------CGCCGGUg---GUAUGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 17126 | 0.69 | 0.937743 |
Target: 5'- cGCGUACAuuGCGGaCCcuACCgcGUGCAgCGCc -3' miRNA: 3'- -UGUAUGUuuCGCC-GG--UGG--UAUGU-GCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 116423 | 0.71 | 0.902307 |
Target: 5'- -aGUGUAAAGCGGCCGCCGaguugauaAUACGUg -3' miRNA: 3'- ugUAUGUUUCGCCGGUGGUa-------UGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 57144 | 0.71 | 0.89547 |
Target: 5'- gUAUGCAGaagugucggcGGUGGuCCACCuacugucuccGUACACGCg -3' miRNA: 3'- uGUAUGUU----------UCGCC-GGUGG----------UAUGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 46261 | 0.71 | 0.888375 |
Target: 5'- -aAUACGAaaguguGGCGGUCACCA-ACGCGg -3' miRNA: 3'- ugUAUGUU------UCGCCGGUGGUaUGUGCg -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 25862 | 0.72 | 0.857509 |
Target: 5'- uGCAUAguGAGCuggacGCCGCCGUAaauuguCACGCg -3' miRNA: 3'- -UGUAUguUUCGc----CGGUGGUAU------GUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 70481 | 0.73 | 0.785344 |
Target: 5'- cACAaAC-AGGCGGUCGCCGU-CACGUu -3' miRNA: 3'- -UGUaUGuUUCGCCGGUGGUAuGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 78952 | 0.73 | 0.785344 |
Target: 5'- -uGUGCAAGGUGGUgGCCAacaacggaucgACGCGCa -3' miRNA: 3'- ugUAUGUUUCGCCGgUGGUa----------UGUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 110274 | 0.75 | 0.724458 |
Target: 5'- cACGUACA---CGGCCACCcuagAUugGCGCa -3' miRNA: 3'- -UGUAUGUuucGCCGGUGG----UAugUGCG- -5' |
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11179 | 5' | -50.2 | NC_002816.1 | + | 87701 | 0.75 | 0.713934 |
Target: 5'- cGCGUAUAAuaauugauAGUGGCCugCAUGCGCu- -3' miRNA: 3'- -UGUAUGUU--------UCGCCGGugGUAUGUGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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