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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 11181 | 3' | -49 | NC_002816.1 | + | 54150 | 0.67 | 0.989924 |
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Target: 5'- uAUGGACCUGCUGuGACuuCGUCucuGUGGGc -3' miRNA: 3'- -UAUCUGGAUGAC-CUGu-GUGGu--UAUCC- -5' |
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| 11181 | 3' | -49 | NC_002816.1 | + | 99968 | 0.67 | 0.992402 |
|
Target: 5'- uUGGGCacauUugUGGGC--GCCAAUAGGg -3' miRNA: 3'- uAUCUGg---AugACCUGugUGGUUAUCC- -5' |
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| 11181 | 3' | -49 | NC_002816.1 | + | 104596 | 0.68 | 0.98507 |
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Target: 5'- aAUAGGCCcACUuGACACACCAc---- -3' miRNA: 3'- -UAUCUGGaUGAcCUGUGUGGUuaucc -5' |
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| 11181 | 3' | -49 | NC_002816.1 | + | 94805 | 0.7 | 0.963579 |
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Target: 5'- --uGGCCaACUGGcCGCGgCGAUAGGc -3' miRNA: 3'- uauCUGGaUGACCuGUGUgGUUAUCC- -5' |
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| 11181 | 3' | -49 | NC_002816.1 | + | 121587 | 0.71 | 0.94239 |
|
Target: 5'- uGUGGAgcgccagCUACUcGGAUACACCAAUGGa -3' miRNA: 3'- -UAUCUg------GAUGA-CCUGUGUGGUUAUCc -5' |
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| 11181 | 3' | -49 | NC_002816.1 | + | 73320 | 0.74 | 0.82996 |
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Target: 5'- -aAGACCaUugUGGACAgCACCAGgauGGa -3' miRNA: 3'- uaUCUGG-AugACCUGU-GUGGUUau-CC- -5' |
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| 11181 | 3' | -49 | NC_002816.1 | + | 50463 | 1.1 | 0.008767 |
|
Target: 5'- aAUAGACCUACUGGACACACCAAUAGGa -3' miRNA: 3'- -UAUCUGGAUGACCUGUGUGGUUAUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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