Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11181 | 5' | -50.5 | NC_002816.1 | + | 31747 | 0.7 | 0.902515 |
Target: 5'- -cCCUCCcuCACACCCAUgUuguAGUACAg -3' miRNA: 3'- uuGGAGGuuGUGUGGGUAgG---UUAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 86862 | 0.73 | 0.784421 |
Target: 5'- cACCUCCAACGCGCUauaAUCCAc---- -3' miRNA: 3'- uUGGAGGUUGUGUGGg--UAGGUuaugu -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 75255 | 0.74 | 0.733443 |
Target: 5'- cGCCUCCAACACGCCCu----GUAUAa -3' miRNA: 3'- uUGGAGGUUGUGUGGGuagguUAUGU- -5' |
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11181 | 5' | -50.5 | NC_002816.1 | + | 50425 | 1.07 | 0.008588 |
Target: 5'- aAACCUCCAACACACCCAUCCAAUACAa -3' miRNA: 3'- -UUGGAGGUUGUGUGGGUAGGUUAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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