miRNA: 3'- gAAAAUUGUGGU---UGCGGU---UGUU-----GCCG- -5'
miRNA: 3'- gaaaAUUGUggUugCGGUUGuuGCCG- -5'
miRNA: 3'- gaaaaUUGugGUU-GCGGu--UGUUGCCG- -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11182 | 5' | -45.4 | NC_002816.1 | + | 121770 | 0.67 | 0.999801 |
|
Target: 5'- aUUUUGACACCAuggGCGCUGuacACAAuccauUGGUg -3' miRNA: 3'- gAAAAUUGUGGU---UGCGGU---UGUU-----GCCG- -5' |
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| 11182 | 5' | -45.4 | NC_002816.1 | + | 121986 | 0.68 | 0.999671 |
|
Target: 5'- ---gUAACGuuGugGCCAGCcuUGGCc -3' miRNA: 3'- gaaaAUUGUggUugCGGUUGuuGCCG- -5' |
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| 11182 | 5' | -45.4 | NC_002816.1 | + | 122481 | 0.68 | 0.999671 |
|
Target: 5'- ----aAACugUAAcCGCCcguACAACGGUg -3' miRNA: 3'- gaaaaUUGugGUU-GCGGu--UGUUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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