miRNA: 3'- gaaaaUUGUGG------UUGCGGU---UGUUGCCG- -5'
miRNA: 3'- gaaaaUUGuGgUUGCGGUUGU--UGC-CG- -5'
miRNA: 3'- gaaaAUUGUGGU--UGCGGuUGUUGCcg -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11182 | 5' | -45.4 | NC_002816.1 | + | 36460 | 0.67 | 0.999883 |
|
Target: 5'- ----cAACACCcuggauGAUGUCAuauGCGACGGUg -3' miRNA: 3'- gaaaaUUGUGG------UUGCGGU---UGUUGCCG- -5' |
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| 11182 | 5' | -45.4 | NC_002816.1 | + | 10369 | 0.67 | 0.999912 |
|
Target: 5'- ----gGGCuCgGGCGCCAACAacACGcGCa -3' miRNA: 3'- gaaaaUUGuGgUUGCGGUUGU--UGC-CG- -5' |
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| 11182 | 5' | -45.4 | NC_002816.1 | + | 30400 | 0.66 | 0.999981 |
|
Target: 5'- ---cUAACACCAccAUGCCcACAAUGa- -3' miRNA: 3'- gaaaAUUGUGGU--UGCGGuUGUUGCcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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