Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11183 | 3' | -47.3 | NC_002816.1 | + | 34428 | 0.66 | 0.999001 |
Target: 5'- -uGGGaAGGUGuuUGCUGAAUUUGGu- -3' miRNA: 3'- uuCUC-UCCAUggACGAUUUGAACUug -5' |
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11183 | 3' | -47.3 | NC_002816.1 | + | 63957 | 0.66 | 0.998769 |
Target: 5'- gGAGAGGGGUguggguucACUUugacGCUGucuCUUGAACa -3' miRNA: 3'- -UUCUCUCCA--------UGGA----CGAUuu-GAACUUG- -5' |
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11183 | 3' | -47.3 | NC_002816.1 | + | 556 | 0.66 | 0.998166 |
Target: 5'- -cGAGAGcGUACUgcaUGgUGAACUUGuAGCa -3' miRNA: 3'- uuCUCUC-CAUGG---ACgAUUUGAAC-UUG- -5' |
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11183 | 3' | -47.3 | NC_002816.1 | + | 58304 | 1.08 | 0.014346 |
Target: 5'- cAAGAGAGGUACCUGCUAAACUUGAACu -3' miRNA: 3'- -UUCUCUCCAUGGACGAUUUGAACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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