Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11184 | 5' | -52.8 | NC_002816.1 | + | 63182 | 0.66 | 0.947667 |
Target: 5'- cGggCGGACGAGGUGGauuguGguUCGCg-- -3' miRNA: 3'- aCa-GCCUGUUUCACCg----CguAGCGaua -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 17707 | 0.66 | 0.943025 |
Target: 5'- aUGUUGGGCAgagcguuaaccaGAGUGGUGaCGUCGg--- -3' miRNA: 3'- -ACAGCCUGU------------UUCACCGC-GUAGCgaua -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 71021 | 0.66 | 0.938123 |
Target: 5'- cGUgGGGauguGUGGCGUugaGUCGCUGg -3' miRNA: 3'- aCAgCCUguuuCACCGCG---UAGCGAUa -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 106676 | 0.67 | 0.932959 |
Target: 5'- uUGUCGGAUAuuGGUcuguauaGCGCGUCGCc-- -3' miRNA: 3'- -ACAGCCUGUu-UCAc------CGCGUAGCGaua -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 110438 | 0.67 | 0.929188 |
Target: 5'- uUGUCGGugAcugugcgccaaucuaGGGUGGCcguguacGUGUCGCUGc -3' miRNA: 3'- -ACAGCCugU---------------UUCACCG-------CGUAGCGAUa -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 100807 | 0.68 | 0.896453 |
Target: 5'- aGUgGGA-GGAGUGGCGCgaaugucuaauGUUGCUGUg -3' miRNA: 3'- aCAgCCUgUUUCACCGCG-----------UAGCGAUA- -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 99137 | 0.68 | 0.867013 |
Target: 5'- cGUCGGGCGA--UGGCGCAcuUgGCa-- -3' miRNA: 3'- aCAGCCUGUUucACCGCGU--AgCGaua -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 87383 | 0.69 | 0.859055 |
Target: 5'- uUGUUGaGCAAGGUGGUGUAUUGUUu- -3' miRNA: 3'- -ACAGCcUGUUUCACCGCGUAGCGAua -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 77848 | 0.7 | 0.797463 |
Target: 5'- -aUCGGACAGuGUGGUGC--CGCUGa -3' miRNA: 3'- acAGCCUGUUuCACCGCGuaGCGAUa -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 106805 | 0.7 | 0.787929 |
Target: 5'- aGUUGGACGggagacgcagccGAGUGGCGCGcacCGCa-- -3' miRNA: 3'- aCAGCCUGU------------UUCACCGCGUa--GCGaua -5' |
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11184 | 5' | -52.8 | NC_002816.1 | + | 80854 | 1.06 | 0.006536 |
Target: 5'- uUGUCGGACAAAGUGGCGCAUCGCUAUc -3' miRNA: 3'- -ACAGCCUGUUUCACCGCGUAGCGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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