Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11186 | 3' | -51.3 | NC_002816.1 | + | 45679 | 0.66 | 0.981438 |
Target: 5'- ----cUGGUGuuuaGUGUGgUGUUGAGCGa -3' miRNA: 3'- guuaaGCCAC----UACACgACGACUCGCc -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 36434 | 0.67 | 0.968105 |
Target: 5'- ----aCGGUGAcuauUUGCUGAGCGGc -3' miRNA: 3'- guuaaGCCACUacacGACGACUCGCC- -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 77991 | 0.69 | 0.934641 |
Target: 5'- ----aCGGUGAUGUuuUGUguaGAGCGGc -3' miRNA: 3'- guuaaGCCACUACAcgACGa--CUCGCC- -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 86536 | 0.69 | 0.923708 |
Target: 5'- ---gUUGGUGAUGUcGUUGgUGAGCa- -3' miRNA: 3'- guuaAGCCACUACA-CGACgACUCGcc -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 11498 | 0.69 | 0.923708 |
Target: 5'- ----aCGGaGA-GUGCUGCggUGGGCGGa -3' miRNA: 3'- guuaaGCCaCUaCACGACG--ACUCGCC- -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 36744 | 0.69 | 0.90534 |
Target: 5'- --uUUCGGUGAaagUGUGCggagGUgUGGGUGGa -3' miRNA: 3'- guuAAGCCACU---ACACGa---CG-ACUCGCC- -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 98061 | 0.7 | 0.87801 |
Target: 5'- gCAAaUCGGUG-UGUGUccaguguuaucaagaGCUGAGCGGu -3' miRNA: 3'- -GUUaAGCCACuACACGa--------------CGACUCGCC- -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 84217 | 0.71 | 0.85366 |
Target: 5'- ---aUgGGUGAUGUgGUUGUcggUGAGCGGu -3' miRNA: 3'- guuaAgCCACUACA-CGACG---ACUCGCC- -5' |
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11186 | 3' | -51.3 | NC_002816.1 | + | 86911 | 1.12 | 0.004057 |
Target: 5'- cCAAUUCGGUGAUGUGCUGCUGAGCGGg -3' miRNA: 3'- -GUUAAGCCACUACACGACGACUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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