Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11186 | 5' | -54.2 | NC_002816.1 | + | 86876 | 1.08 | 0.004024 |
Target: 5'- uGUCCACCAACAGACACCUCCAACGCGc -3' miRNA: 3'- -CAGGUGGUUGUCUGUGGAGGUUGCGC- -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 45892 | 0.74 | 0.537942 |
Target: 5'- -aCCugCAGCGGACACUguaCCGGCGaCGg -3' miRNA: 3'- caGGugGUUGUCUGUGGa--GGUUGC-GC- -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 99605 | 0.73 | 0.589578 |
Target: 5'- uGUCgGCCAACAGuGCACUcgCCAACGUu -3' miRNA: 3'- -CAGgUGGUUGUC-UGUGGa-GGUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 60827 | 0.73 | 0.600031 |
Target: 5'- uGUCCGCC-AUGGACAgCaaCCAACGCGa -3' miRNA: 3'- -CAGGUGGuUGUCUGUgGa-GGUUGCGC- -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 15430 | 0.71 | 0.683862 |
Target: 5'- uUCCAucuUCAACAGACGCuCUUCGugGCu -3' miRNA: 3'- cAGGU---GGUUGUCUGUG-GAGGUugCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 92446 | 0.7 | 0.735081 |
Target: 5'- -cCCACCAGuCAGAUuCCcgCCGACGCc -3' miRNA: 3'- caGGUGGUU-GUCUGuGGa-GGUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 75762 | 0.7 | 0.745082 |
Target: 5'- -cCCACCGAC-GACGCCcaaUCCAccaaAUGCGa -3' miRNA: 3'- caGGUGGUUGuCUGUGG---AGGU----UGCGC- -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 8658 | 0.7 | 0.774419 |
Target: 5'- -cCCGCCcucuuGCaAGAUACuCUCCAGCGCc -3' miRNA: 3'- caGGUGGu----UG-UCUGUG-GAGGUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 61284 | 0.69 | 0.82051 |
Target: 5'- aUCCuCCGuCGGugGCUcCCAACGCGu -3' miRNA: 3'- cAGGuGGUuGUCugUGGaGGUUGCGC- -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 46087 | 0.69 | 0.802552 |
Target: 5'- -cCCACUuuGGCGGGCACCguucguuugCuCAGCGCGa -3' miRNA: 3'- caGGUGG--UUGUCUGUGGa--------G-GUUGCGC- -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 105546 | 0.69 | 0.829223 |
Target: 5'- cGUCCACCGAucacCAGACAUacgCUCCAccacCGCc -3' miRNA: 3'- -CAGGUGGUU----GUCUGUG---GAGGUu---GCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 11711 | 0.69 | 0.802552 |
Target: 5'- -gCCACC-GCGGACACCUUCAA-GUa -3' miRNA: 3'- caGGUGGuUGUCUGUGGAGGUUgCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 466 | 0.68 | 0.854196 |
Target: 5'- aUCCGCCAACaAGACcCCUacuACGUGg -3' miRNA: 3'- cAGGUGGUUG-UCUGuGGAgguUGCGC- -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 74790 | 0.68 | 0.854196 |
Target: 5'- aUCCAguuuacgcCCGACGGACGCg-CCAACGUu -3' miRNA: 3'- cAGGU--------GGUUGUCUGUGgaGGUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 50168 | 0.68 | 0.869799 |
Target: 5'- uGUCCACCAGCAcACACUguuuguguugggUgUAGCGCa -3' miRNA: 3'- -CAGGUGGUUGUcUGUGG------------AgGUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 27164 | 0.67 | 0.904782 |
Target: 5'- aUCCACC-ACAGcCAUCUCCGcaGCa -3' miRNA: 3'- cAGGUGGuUGUCuGUGGAGGUugCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 42298 | 0.67 | 0.877266 |
Target: 5'- uUCCGCCgAACAaauacGACGCCUCacacaaauCAACGCc -3' miRNA: 3'- cAGGUGG-UUGU-----CUGUGGAG--------GUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 31367 | 0.66 | 0.928365 |
Target: 5'- --aCGCCAcauACGGAUACCgaaaCAGCGCu -3' miRNA: 3'- cagGUGGU---UGUCUGUGGag--GUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 30682 | 0.66 | 0.925083 |
Target: 5'- -gCCGCCGGCu-ACACCagauuuucguacucgUCCGGCGCa -3' miRNA: 3'- caGGUGGUUGucUGUGG---------------AGGUUGCGc -5' |
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11186 | 5' | -54.2 | NC_002816.1 | + | 67239 | 0.66 | 0.922845 |
Target: 5'- -aCCACCAACGuuguccgcauCACUgaaUCCGGCGCGu -3' miRNA: 3'- caGGUGGUUGUcu--------GUGG---AGGUUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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