Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11187 | 3' | -52.4 | NC_002816.1 | + | 47289 | 0.66 | 0.967104 |
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Target: 5'- --aGUugAUGGGCGCGCuCGacccggUGACAa -3' miRNA: 3'- uuaCAuaUACCCGCGCG-GCaa----GCUGU- -5' |
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| 11187 | 3' | -52.4 | NC_002816.1 | + | 17082 | 0.66 | 0.947667 |
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Target: 5'- -cUGUGUAauUGGGCgucguucaaGCGCaUGUUUGACAc -3' miRNA: 3'- uuACAUAU--ACCCG---------CGCG-GCAAGCUGU- -5' |
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| 11187 | 3' | -52.4 | NC_002816.1 | + | 82645 | 0.67 | 0.943025 |
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Target: 5'- --gGUGUcUGuGUGCGCCGUcCGGCAc -3' miRNA: 3'- uuaCAUAuACcCGCGCGGCAaGCUGU- -5' |
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| 11187 | 3' | -52.4 | NC_002816.1 | + | 79677 | 0.68 | 0.909667 |
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Target: 5'- ----gAUAUGcGUGUGCUGUUCGGCAa -3' miRNA: 3'- uuacaUAUACcCGCGCGGCAAGCUGU- -5' |
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| 11187 | 3' | -52.4 | NC_002816.1 | + | 54749 | 0.76 | 0.485416 |
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Target: 5'- --aGUGUGUGGGCGaGCCGUugUUGGCGu -3' miRNA: 3'- uuaCAUAUACCCGCgCGGCA--AGCUGU- -5' |
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| 11187 | 3' | -52.4 | NC_002816.1 | + | 10518 | 1.06 | 0.006729 |
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Target: 5'- cAAUGUAUAUGGGCGCGCCGUUCGACAc -3' miRNA: 3'- -UUACAUAUACCCGCGCGGCAAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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