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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 1119 | 3' | -55.8 | NC_000942.1 | + | 15077 | 0.66 | 0.503602 |
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Target: 5'- uAGGCGUCGCcgccgucGACGuAGAUCcCCUCg -3' miRNA: 3'- uUCCGCAGCG-------UUGUcUCUGGuGGAGg -5' |
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| 1119 | 3' | -55.8 | NC_000942.1 | + | 1253 | 0.66 | 0.504691 |
|
Target: 5'- cAAGGCuccUGUaaaGACAGAG-UCGCCUCCa -3' miRNA: 3'- -UUCCGca-GCG---UUGUCUCuGGUGGAGG- -5' |
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| 1119 | 3' | -55.8 | NC_000942.1 | + | 7892 | 0.71 | 0.240164 |
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Target: 5'- cGGGCGUCGUuguuGACGaAGACgaguCGCCUCCu -3' miRNA: 3'- uUCCGCAGCG----UUGUcUCUG----GUGGAGG- -5' |
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| 1119 | 3' | -55.8 | NC_000942.1 | + | 16459 | 0.72 | 0.215198 |
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Target: 5'- gAAGGCGUCGUcauGGCGGAcACCGCUcagagUCCu -3' miRNA: 3'- -UUCCGCAGCG---UUGUCUcUGGUGG-----AGG- -5' |
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| 1119 | 3' | -55.8 | NC_000942.1 | + | 12499 | 1.09 | 0.000349 |
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Target: 5'- gAAGGCGUCGCAACAGAGACCACCUCCu -3' miRNA: 3'- -UUCCGCAGCGUUGUCUCUGGUGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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