Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11190 | 5' | -54.5 | NC_002816.1 | + | 11457 | 0.66 | 0.927493 |
Target: 5'- cGGUGAGgagggugUGGUGGugGCuCGACCc-- -3' miRNA: 3'- -CCGCUCaa-----GCCGCCugUG-GUUGGuuu -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 23292 | 0.66 | 0.916113 |
Target: 5'- uGGgGGGaaugUCGGCGGGguCCAGCg--- -3' miRNA: 3'- -CCgCUCa---AGCCGCCUguGGUUGguuu -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 110821 | 0.66 | 0.916113 |
Target: 5'- aGCGAucgcauGUUCuuGGCGGGCGagucccCCGACCAAu -3' miRNA: 3'- cCGCU------CAAG--CCGCCUGU------GGUUGGUUu -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 25056 | 0.66 | 0.912503 |
Target: 5'- gGGCGGGUUCGaaGUGGguuucacugguugacACACUguuAACCAGAg -3' miRNA: 3'- -CCGCUCAAGC--CGCC---------------UGUGG---UUGGUUU- -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 19292 | 0.66 | 0.910047 |
Target: 5'- cGGCGGGUUCauuGGCGGGggacuccuguucCGCCu-CCAu- -3' miRNA: 3'- -CCGCUCAAG---CCGCCU------------GUGGuuGGUuu -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 78878 | 0.67 | 0.860791 |
Target: 5'- cGGCG-GUaugaggacUCGGuCGGugGCCGACUg-- -3' miRNA: 3'- -CCGCuCA--------AGCC-GCCugUGGUUGGuuu -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 61038 | 0.68 | 0.852839 |
Target: 5'- cGGCG-GUUCGacaauGUGGGCACCAuaGCuCAAGu -3' miRNA: 3'- -CCGCuCAAGC-----CGCCUGUGGU--UG-GUUU- -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 17351 | 0.7 | 0.753236 |
Target: 5'- gGGUccgGGGUUgGGCuguGGACACgAACCAGAg -3' miRNA: 3'- -CCG---CUCAAgCCG---CCUGUGgUUGGUUU- -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 67429 | 0.7 | 0.753236 |
Target: 5'- uGGCG-GUgguagCGGUGGGCACUAGCa--- -3' miRNA: 3'- -CCGCuCAa----GCCGCCUGUGGUUGguuu -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 16192 | 0.7 | 0.727227 |
Target: 5'- uGGCGuGGUUCGGCGccGACGaaaucgucuccauacUCAACCAAAa -3' miRNA: 3'- -CCGC-UCAAGCCGC--CUGU---------------GGUUGGUUU- -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 119558 | 0.7 | 0.717058 |
Target: 5'- cGGUGGGguaggCGGCGGgacauuauucgauugGCACgCGACCAAAc -3' miRNA: 3'- -CCGCUCaa---GCCGCC---------------UGUG-GUUGGUUU- -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 8529 | 0.71 | 0.702695 |
Target: 5'- gGGCGGGguggugUCGGCGGAaaACgAGCCGu- -3' miRNA: 3'- -CCGCUCa-----AGCCGCCUg-UGgUUGGUuu -5' |
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11190 | 5' | -54.5 | NC_002816.1 | + | 23506 | 1.09 | 0.003084 |
Target: 5'- cGGCGAGUUCGGCGGACACCAACCAAAg -3' miRNA: 3'- -CCGCUCAAGCCGCCUGUGGUUGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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