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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11192 | 3' | -51.6 | NC_002816.1 | + | 17902 | 0.7 | 0.868406 |
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Target: 5'- cGAGUCuagGGUGACGGCCACagUUugGgGg -3' miRNA: 3'- -CUUAG---CCGCUGCUGGUGa-AAugCgCa -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 29338 | 1.08 | 0.006934 |
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Target: 5'- aGAAUCGGCGACGACCACUUUACGCGUg -3' miRNA: 3'- -CUUAGCCGCUGCUGGUGAAAUGCGCA- -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 32733 | 0.68 | 0.956328 |
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Target: 5'- cAAUCGGCGACGggaccccacgguGCCugUUgaugAgGCGg -3' miRNA: 3'- cUUAGCCGCUGC------------UGGugAAa---UgCGCa -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 63369 | 0.71 | 0.841695 |
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Target: 5'- -uAUUGGUacGACGACCACUUUgucuaggguacaaaACGCGa -3' miRNA: 3'- cuUAGCCG--CUGCUGGUGAAA--------------UGCGCa -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 68316 | 0.66 | 0.975889 |
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Target: 5'- aGAUCGGCGACGucugACCAUgu--UGUGUu -3' miRNA: 3'- cUUAGCCGCUGC----UGGUGaaauGCGCA- -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 74956 | 0.66 | 0.977412 |
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Target: 5'- uAGUUGGCGGaugcguggacaaaGACCACUaugUugGCGg -3' miRNA: 3'- cUUAGCCGCUg------------CUGGUGAa--AugCGCa -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 85797 | 0.67 | 0.970267 |
|
Target: 5'- uGAUCGGUGugGugUugUUUAUaGUGUu -3' miRNA: 3'- cUUAGCCGCugCugGugAAAUG-CGCA- -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 94803 | 0.71 | 0.860565 |
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Target: 5'- -uGUCGGC-ACcACCACUUUGCGCc- -3' miRNA: 3'- cuUAGCCGcUGcUGGUGAAAUGCGca -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 99003 | 0.68 | 0.952246 |
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Target: 5'- -cAUCGccCGACGACCACcacUACGCGc -3' miRNA: 3'- cuUAGCc-GCUGCUGGUGaa-AUGCGCa -5' |
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| 11192 | 3' | -51.6 | NC_002816.1 | + | 99433 | 0.67 | 0.967129 |
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Target: 5'- cAAagGGCGACGACUACacaaaaacgUugGCGa -3' miRNA: 3'- cUUagCCGCUGCUGGUGaa-------AugCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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