Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11194 | 5' | -56.6 | NC_002816.1 | + | 32756 | 0.66 | 0.858476 |
Target: 5'- uGCCugUugaugaGGCGGUGGGUCGGGCAgGu- -3' miRNA: 3'- -UGGugG------CUGUCGCUCAGCUCGUgUga -5' |
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11194 | 5' | -56.6 | NC_002816.1 | + | 34059 | 1.08 | 0.002414 |
Target: 5'- cACCACCGACAGCGAGUCGAGCACACUg -3' miRNA: 3'- -UGGUGGCUGUCGCUCAGCUCGUGUGA- -5' |
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11194 | 5' | -56.6 | NC_002816.1 | + | 98552 | 0.66 | 0.858476 |
Target: 5'- uGCCACCGGCGcCGAGgacucuGUACGCg -3' miRNA: 3'- -UGGUGGCUGUcGCUCagcu--CGUGUGa -5' |
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11194 | 5' | -56.6 | NC_002816.1 | + | 117298 | 0.67 | 0.799263 |
Target: 5'- gGCCACCGAUAGCauacuuUUGAGCAauucCACUu -3' miRNA: 3'- -UGGUGGCUGUCGcuc---AGCUCGU----GUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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