Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11196 | 5' | -62.2 | NC_002816.1 | + | 22133 | 0.66 | 0.565888 |
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Target: 5'- cAGuCACGCagacagauaacaACGCCCggucacguaCGCCCGGUCACg- -3' miRNA: 3'- -UC-GUGUG------------UGCGGG---------GUGGGCCGGUGac -5' |
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| 11196 | 5' | -62.2 | NC_002816.1 | + | 32243 | 0.7 | 0.336942 |
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Target: 5'- gGGCAC-UACGCCUCGgCUGGCCuACUa -3' miRNA: 3'- -UCGUGuGUGCGGGGUgGGCCGG-UGAc -5' |
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| 11196 | 5' | -62.2 | NC_002816.1 | + | 48091 | 0.67 | 0.488812 |
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Target: 5'- uGCGCACAC-UCCCACUCGuGUCAauCUGg -3' miRNA: 3'- uCGUGUGUGcGGGGUGGGC-CGGU--GAC- -5' |
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| 11196 | 5' | -62.2 | NC_002816.1 | + | 55613 | 0.69 | 0.366796 |
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Target: 5'- cGGCACACAUGaUCCCAaCCGGCUcguugaaauaaccGCUGc -3' miRNA: 3'- -UCGUGUGUGC-GGGGUgGGCCGG-------------UGAC- -5' |
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| 11196 | 5' | -62.2 | NC_002816.1 | + | 56495 | 1.07 | 0.00078 |
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Target: 5'- gAGCACACACGCCCCACCCGGCCACUGu -3' miRNA: 3'- -UCGUGUGUGCGGGGUGGGCCGGUGAC- -5' |
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| 11196 | 5' | -62.2 | NC_002816.1 | + | 69524 | 0.66 | 0.536503 |
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Target: 5'- uGCGCAUACGagaCCCCGCCaucuauGCCGCa- -3' miRNA: 3'- uCGUGUGUGC---GGGGUGGgc----CGGUGac -5' |
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| 11196 | 5' | -62.2 | NC_002816.1 | + | 92373 | 0.68 | 0.434369 |
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Target: 5'- aGGUGCuuuCGCCCaaACCCGGCUACUa -3' miRNA: 3'- -UCGUGuguGCGGGg-UGGGCCGGUGAc -5' |
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| 11196 | 5' | -62.2 | NC_002816.1 | + | 117532 | 0.69 | 0.406806 |
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Target: 5'- uGGCGCACAUGCCCgGCgacgagcgauaGGCUACUa -3' miRNA: 3'- -UCGUGUGUGCGGGgUGgg---------CCGGUGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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